ABSTRACT: Surface treatments are widely used techniques for improving the biocompatibility of polymeric materials. Among these treatments, the kINPen® IND cold plasma jet has stood out as an efficient and promising option. The Acrylonitrile Butadiene Styrene (ABS) polymer has favourable mechanical and chemical properties for application in the medical field. However, its low biocompatibility limits its use in certain applications. Therefore, this study presents the application of this treatment to improve its biocompatibility and the cell adhesion assessments. ABS samples were manufactured from 3D printing via FDM, using the GTMax3D Core H4 printer, and subjected to treatment with kINPen® IND. Analysis of wettability using the Ramé-Hart 110-00 goniometer showed an increase in the material's hydrophilicity. The cell adhesion tests with MEF L929 cells showed no significant change in the number of adhered cells in despite of hydrophilicity. Anyway, the results of cold plasma treatment showed that it has a great potential for application in biomaterials for various areas, such as the medical industry and the production of biomedical devices. More cell studies are needed to assess the effectiveness and safety of this method on different materials and applications.